chimeric synthetic peptides for infectious diseases immunodiagnostic

chimeric synthetic peptides for infectious diseases immunodiagnostic

CHIMERIC SYNTHETIC PEPTIDES FOR THE IMMUNODIAGNOSTIC OF
INFECTIOUS DISEASES
Milenen Hernández Marin1*, Chryslaine Rodríguez Tanty 2, Lilliam Pozo Peña 1,
Yadaris Márquez Bocalandro 1, María Elena Selles León 1, David Higginson Clarke 2,
Martha Amat Arenas 1, Grisell Martínez Ramos 1, Idialis Hernández Spengler 1 , Jeny
González Pérez3, Yaranaima Morán Peraza1
1 Centro de Inmunoensayo, Calle 134 y ave 25, Cubanacán, Playa, La Habana, Cuba.
2 Centro Nacional de Neurociencias de Cuba, La Habana, Cuba.
3 Facultad de Biología, Universidad de la Habana, Cuba
* e-mail: [email protected]
Six chimeric synthetic peptides (Q(C-1), Q(C-1-1), Q(C-15), Q(C-15-1), Q(C-16) and
Q(C-16-1)), corresponding to regions of immunologically important proteins of HIV-1,
gp41 transmembrane protein and 120 envelope protein, four chimeric peptides (Q-1, Q2, Q-3, Q-4) incorporating two sequences of the core p19 and the envelope gp46
proteins of the HTLV-I, six chimeric peptides (Cha-1, Cha-2, Cha-3, Cha-4, Cha-5 and
Cha-6), corresponding to immunodominant regions of the Trypanosoma cruzi, were
synthesized by conventional solid-phase peptide synthesis. Those peptides were
arranged in a way that permits to obtain different combinations of chimeric peptides and
separated by two glycine residues as spacers arm. The antigenic activities of the
chimeric
synthetic
peptides
were
evaluated
by
UltramicroEnzyme-linked
immunosorbent assay (UMELISA) by using positive samples of HIV-1/2, HTLV-I/II
and Chagas disease. The efficacy of the chimeric peptides in solid-phase immunoassays
was compared with the monomeric peptides. Data demonstrated that the chimeric
peptides were the most reactive because detected antibodies to infectious agents
efficiently. This may be related to peptide adsorption to the solid surface and epitope
accessibility to the antibodies. The results demonstrated the utility of the chimeric
peptides, as antigens, in the immunodiagnostic of these infectious diseases.