Curriculum 2012 Alberto Miranda

CURRICULUM VITAE
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Alberto Miranda Bedate
22/11/1982
Spanish
Veldhoeve 94 3438 LM Nieuwegein Nederland
06-26418856
[email protected]
http://nl.linkedin.com/pub/alberto-miranda-bedate/42/989/809
Professional profile:
Pharmacist, researcher
• Motivated pharmacist / researcher, specialist in biochemistry/molecular cell biology
• Excellent aptitudes in design, management and performance of experiments involving
molecular biology, cell culture and/or transgenic rodent models.
Main skills/abilities:
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Molecular biology techniques: RT-PCR, cloning, gene delection, sequencing,
microarray, western blot, bioinformatics…
Cell culture techniques: maintenance of stem cells, differentiation protocols, antibody
inhibition experiments, sampling…
Animal (rodent) handling skills
Bioinformatics
Ability to work in team as well as independently
Excellent communication and interpersonal skills
Previous professional activities:
2012
Research collaborator Animal Reproduction Department-INIA (Spain)
(January 2012 – to the present)
Main research area: Epigenetics of mice produced by in vitro sub-optimal
culture. Microarray analysis of developmental epigenetics.
2010-2011
Research staff Animal Reproduction Department-INIA,
Spanish Ministry of Science and Innovation (18 months)
Main research area: Transcriptional profile of mouse embryonic stem cells.
Microarray analysis of developmental epigenetics. Prion protein involvement in
the development of sperm cells.
2005-2010
PhD Student/ Research staff (PhD grant of the Spanish Ministry
of Science and Innovation (FPU)):
Animal Reproduction Department-INIA, Spanish Ministry of Science and
Innovation (2009-2010, 12 months)
Main research area: Involvement of the prion protein in the pluripotency
maintenance of mouse embryonic stem cells (Transcriptional analysis).
Production and maintenance of transgenic mouse lines.
CISA-INIA (Centre for Investigation in Animal Health), Spanish Ministry of
Science and Innovation (2007-2009, 18 months)
Main research area: Susceptibility to scrapie of several transgenic mouse
models carrying different polimorfisms of the prion protein. Production and
maintenance of transgenic mouse lines.
Microbiology II Department, Faculty of Pharmaceutical Sciences, Universidad
Complutense de Madrid, Spain (2005-2007, 24 months)
Main research area: MAP kinases involvement in oxidative stress resistance of
the pathogenic fungus C. albicans
2005
Pharmacist Pharmacy (Madrid, Spain) (6 months)
2004-2005
Colaboration Grant Microbiology II Department, Faculty of
Pharmaceutical Sciences, Universidad Complutense de Madrid,
Spain (12 months)
Academic Background:
2011
PhD in Microbiology and Parasitology
Universidad Complutense de Madrid
C
PhD title: “Funcionalidad de la PrP en células troncales embrionarias de ratón
C
y susceptibilidad diferencial a Scrapie de sus polimorfismos en cabra” (PrP
functionality in
mouse Embryonic Stem Cells and differential susceptibility of its
polymorphisms in Goat).
2007
Diploma de Estudios Avanzados (DEA) (Equivalent to Msc-degree).
Universidad Complutense de Madrid (Madrid, Spain)
2005
B.S Degree in Pharmaceutical Sciences
Universidad Complutense de Madrid, Spain
Language skills:
• Spanish (Native)
• English (writing and speaking: fluent)
• Dutch (basic conversation and learning)
Congress: 13 oral and poster participations (2 national and 11 international meetings)
Publications: 10 (7 + 3 Book Chapters)
1. Miranda A, Pericuesta E, Ramirez MA, Gutierrez-Adan A. Prion protein in ESC
regulation (Review) (2013) Reproduction (In press)
2. Miranda A, Gutierrez-Adan A. Sexual dimorphism adaptation to microsomic in inbred
mice at birth (2012) (In press).
3. Alexandra Calle, Alberto Miranda, Raul Fernandez-Gonzalez, Eva Pericuesta, Ricardo
Laguna and Alfonso Gutierrez-Adan (2012). Male Mice Produced by In Vitro Culture
Have Reduced Fertility and Transmit Organomegaly and Glucose Intolerance to Their
Male Offspring. Biology Of Reproduction. (BOR Papers in Press. Published on May
30, 2012 as DOI:10.1095/biolreprod.112.100743)
4. Miranda A, Pericuesta E, Ramirez MA, Gutierrez-Adan A. Prion protein expression
regulates embryonic stem cell pluripotency and differentiation. PLoS One
2011;6(4):e18422.
5. A. Miranda, E. Pericuesta, MA Ramirez and A. Gutierrez-Adan (2011). Prion protein in
ESC regulation. PRION 2011 Jul 1;5(3). PMID: 21814032.
6. S Pérez-Cerezales, A Miranda and A Gutiérrez-Adán (2011) Comparison of four
methods to evaluate sperm DNA integrity between mouse caput and cauda epididymis.
Asian Journal of Andrology (Asian J Androl. 2012 Mar;14(2):335-7. doi:
10.1038/aja.2011.119. Epub 2011 Oct 17).
7. P Bermejo-Alvarez, E. Pericuesta, A. Miranda, C. Frutos, S. Pérez-Cerezales, AC
Lucio, D Rizos, and A Gutierrez-Adan (2011) New challenges in the analysis of gene
transcription in bovine blastocysts. Reprod Domest Anim. 2011 Sep;46 Suppl 3:2-10.
doi: 10.1111/j.1439-0531.2011.01842.x.
8. Gutierrez-Adán, A. Calero, A. Calle, E. Pericuesta, R. Fernández-González, A.
Miranda, R. Laguna, P. Ramos, C. Frutos, and M. A. Ramírez (2010) Fronteras en la
producción de animales transgénicos. XXXIII Curso Internacional de Reproducción
Animal Compendio de Conferencia. Ed Vision Libros. ISBN: 978-84-3117696. pp:33-45.
9. Gutierrez-Adán, A. Calero, A. Calle, E. Pericuesta, R. Fernández-González, A.
Miranda, R. Laguna, P. Ramos, C. Frutos, and M. A. Ramírez (2010) Efectos a largo
plazo de la producción in vitro de embriones bovinos. XXXIII Curso Internacional de
Reproducción Animal Compendio de Conferencia. Ed Vision Libros. ISBN: 978-843117696. pp:307-316.
10. R Fernández-González, P Bermejo-Álvarez, M Pérez-Crespo, A Miranda, R Laguna, C
Frutos and A Gutiérrez-Adán (2010) Chapter 10. The Use of Intracytoplasmic Sperm
Injection (ICSI) for Gene Transfer in Mice. In: Sperm-mediated Gene Transfer:
Concepts and Controversies. Kevin Smith (Ed). © 2010 Bentham Science Publishers
Ltd. 146-159.
Reference Researchers:
SURNAME: Gutierrez Adán
FIRST NAME: Alfonso
POSITION: P.I.
DEPARTMENT: Animal Reproduction
UNIVERSITY/ORGANIZATION: INIA
ADDRESS: Ctra. La Coruña s/n, Km 5.9
CITY: Madrid
COUNTRY: Spain
TELEPHONE: +34 913473768
E-MAIL: [email protected]
GENDER: Male
POST CODE: 28040
SURNAME: Manzano Román
FIRST NAME: Raúl
GENDER: Male
POSITION: PostDoctoral
DEPARTMENT: Animal Pathology
UNIVERSITY/ORGANIZATION: Agrobiology and Natural Resources Institute (IRNASA-CSIC)
ADDRESS: Cordel de Merinas, 40
CITY: Salamanca
COUNTRY: Spain
POST CODE: 37008
TELEPHONE: +34 923219606 Ext 214
E-MAIL: [email protected]