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C
HM2 and HM2 TUVO:
Next generation of High-Mass MALDI Retrofit Systems
X
OVAL
Introduction
CovalX introduces its next generation of High-Mass
MALDI detection systems based on unique conversion
dynode technology. HM2 and HM2 TUVO High-Mass
systems allow unmatched detection of macromolecular ions up to 1.2 MDa with nM sensitivity. The HM2 system
is the new reference for the detection of high molecular
weight macromolecules. HM2 TUVO offers the same
features of HM2 with the additional capability to analyse
ions in both positive and negative mode. Choose the
HM2 system that best suit your needs!
HM2 TUVO system retrofit on an ABI SCIEX MALDI ToF/ToF 5800
CovalX iPod Touch Controller
for HM2 and HM2 TUVO.
CovalX's HM2 and HM2 TUVO can be controlled wirelessly using the CovalX's iPod TOUCH application. To switch
between High-Mass detection and standard detection
or between positive and negative ion detection
modes*, simply press a button on the touch screen of
your CovalX's iPod controller.
*option available for HM2 TUVO only
IgM2+
486.63 kDa
2,000
MALDI ToF MS Analysis
Covalx HM2 detector
300 nM
1,500
Intensity
IgM+
973.26 kDa
1,000
500
0
400
HM2 and HM2 TUVO: High-Mass retrofit system
for every standard MALDI ToF mass spectrometer
Applied Biosystems/MDS Sciex
MALDI ToF/ToF 5800
MALDI ToF/ToF 4800
MALDI ToF/ToF 4700
Voyager series
Bruker
Biflex and Reflex series
Autoflex series
Ultraflex series
IgM3+
324.42 kDa
200
Addition of a second detector in the linear mode.
CovalX HM2 and HM2 TUVO retrofit systems do not
change any of the previous features of your MALDI ToF
instrument. After retrofit, the standard detector in the
linear mode is not removed. Using CovalX's iPod
controller the operator can switch between detectors
within seconds.
600
800
m/z (kDa)
1,000
1,200
1,400
Figure 1. High-Mass MALDI ToF mass spectra of the immunoglobulin
IgM (1.4 µM). The mono-charged pseudo-molecular ions of IgM are
detected with m=973.26 kDa.
Shimadzu
Axima CFR / CFR plus
Axima Assurance
Axima Confidence
Axima Performance
C o va l X AG - Te c h n o p a r k s t r a s s e 1 - C H - 8 0 0 5 Z ü r i c h - S wi t z e r l a n d
P h o n e : + 4 1 / ( 0 ) 4 4 6 3 3 7 8 9 7 - E - m a i l : i n f o @ c o v a l x . c o m - w w w. c o v a l x . c o m
COVALX
Oustanding sensitivity for high molecular
weight macromolecules
OFF
Standard detector
CovalX
High Mass System
ON
The HM2 and HM2 TUVO High-Mass detector retrofit
systems allow one to reach outstanding sensitivity for
high-molecular weight macromolecules. Complex
mixtures of proteins can be detected with low saturation. By switching between the standard detector and
the HM2, the operator can easily observe the performance of the High-Mass detector for high-molecular
weight macromolecules.
Flight tube
MALDI source
20000
A
20000
66.5 kDa
High Mass System
Standard detector
15000
BSA
10 µM
133.5 kDa
2BSA
Intensity
Intensity
10000
40 insulins
High Mass System
Standard detector
15000
Insulin
50 µM
10000
B
BSA
198.3 kDa
5000
5000
264.6 kDa
229.3 kDa
4BSA
0
0
50
100
150
200
250
m/z (kDa)
300
350
400
100
400
500
600
D
High Mass System
Standard detector
IgG
IgM
1.2 µM
965.4 kDa
2 IgG
1,000
IgM3+
Intensity
3 IgG
10000
High Mass System
Standard detector
IgM2+
1,500
IgG
10 µM
301.2 kDa
20000
300
m/z (kDa)
C
40000
30000
200
2,000
150.9 kDa
Intensity
3BSA
IgM+
500
452.4 kDa
0
0
100
200
300
400
m/z (kDa)
500
600
700
Figure 2. Comparison between standard detection (red) and HM2
high-mass detection (blue) for 4 proteins (A: Insulin, B: BSA, C: IgG and
D: IgM). Each sample is analyzed from the same spot under identical
instrument settings by only rapidly switching between each detector
using the HM2 controller. The sensitivity of HM2 for high-molecular
weight macromolecules allows the detection of a large number of
insulin, BSA and IgG clusters formed during the MALDI ionization
process. This demonstrates not only the sensitivity of the HM2 detector
but the lack of saturation under abundant ion signals.
200
400
600
800
m/z (kDa)
1,000
1,200
1,400
HM2 High-Mass Detector Retrofit System
Active area
Acceleration voltage
Gain voltage
Mass range
Response time
Resolution
Limit of detection
19 mm
Typically 20 kV
Up to 4.5 kV
Up to 1.2 MDa
350 ns
180 at 200 kDa
300 nM / IgM (978 kDa)
C o va l X AG - Te c h n o p a r k s t r a s s e 1 - C H - 8 0 0 5 Z ü r i c h - S wi t z e r l a n d
P h o n e : + 4 1 / ( 0 ) 4 4 6 3 3 7 8 9 7 - E - m a i l : i n f o @ c o v a l x . c o m - w w w. c o v a l x . c o m