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XX Congreso Nacional de Ingeniería Bioquímica IX Congreso Internacional de Ingeniería Bioquímica XIV Jornadas Científicas de Biomedicina y Biotecnología Molecular Veracruz, México 16-18, Marzo 2016 SPONGE CAKE’S QUALITY DEFINED BY ITS WHEY PROTEIN COMPOSITION AND HEATING RATES DURING BAKING Díaz-Ramírez Mayraa, Calderón-Domínguez Georginab, García-Garibay Marianoa, Jiménez-Guzmán Juditha, Villanueva-Carvajal Adrianad, SalgadoCruz Ma. de la Pazc, Domínguez-López Aureliod a Departamento de Ciencias de la Alimentación. División de Ciencias Biológicas y de la Salud. Universidad Autónoma Metropolitana, Unidad Lerma. Av. Hidalgo Poniente 46, Col. La Estación, Lerma de Villada, Estado de México. 52006. México. b Escuela Nacional de Ciencias Biológicas. Instituto Politécnico Nacional. Carpio y Plan de Ayala, Casco de Sto. Tomás, México, D.F. 11340. México. c Consejo Nacional de Ciencia y Tecnología. Insurgentes Sur 1582, Col. Crédito Constructor, Benito Juárez, Ciudad de México, Distrito Federal, 03940, México d Facultad de Ciencias Agrícolas, Universidad Autónoma del Estado de México, Campus Universitario “El Cerrillo” A.P. 435, Toluca, Estado de México C.P. 50200, México Whey protein isolate is commonly used in food products due to its functional properties, such as foaming and water binding capacity, emulsifying and gel formation ability. Besides, sponge cake is one of the most consumed cakes by the bakery industry due to its porous structure. The aim of the present study was to determine the sponge cake’s quality, defined as the relation between heating rates during baking and the changes on the batter’s kinematic viscosity, via the partial replacement of egg white protein (EWP) by whey protein isolate (WPI). Results showed two heating rates during sponge cake baking; the first one (up to the first twenty minutes, reaching a temperature close to 90°C) was not affected by the addition of WPI, but the second heating rate increased significantly at 12.5% and 25% substitution (0.281°C/min and 0.236°C/min respectively). An opposite effect has been observed at 50% and 100% WPI substitution, where values decreased significantly from the observed in the control sample (-0.013°C/min and 0.66°C/min respectively). The initial kinematic viscosity of the batter caused variations in the temperature rise of it and statistical differences (P<0.05) in the cake’s physical properties like specific volume, moisture, texture and appearance were observed. Therefore, a substitution of 12.5% and 25% showed no significant differences on the quality parameters evaluated when compared to control sample but the total replacement of egg white protein by whey protein isolate resulted in a less quality sponge cake. Key words: Sponge Cake; Whey Protein Isolate; Heating Rate Mar del Norte No. 5, Col. San Álvaro Azcapotzalco C. P. 02090, México, D. F. Tel. y Fax: 5623 3088 email: [email protected], [email protected]